Blue pippin size selection
Web39 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the next-gen sequencing... WebThe Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation …
Blue pippin size selection
Did you know?
WebJun 19, 2013 · This instrument allows for tight size selection of DNA samples, effectively making the peak of the size distribution much more narrow. With regard to PacBio sequencing, a narrow peak lessens the problem of preferential loading of short fragments, leading to much longer ‘reads of insert’. ... It will be nice blue pippin give us better yield ... Web21 hours ago · The genome size, number of ... we used a single-step drug selection ... DNA integrity was evaluated using Blue Pippin pulse gel and the DNA was then used for library preparation using Pacific ...
Web下列发表的文章采用美国Sage Science公司的 Pippin Prep和Blue Pippin ... They showed that Pippin size selection was required to generate usable libraries, even down to sub-nanogram input. Results were comparable to results from … WebMinimum sample amount/lane – 15 ng Maximum sample amount/lane – 1.5 µg Electrophoresis voltage – 75V constant, 75V pulsed field Optical Detection – 470 nm …
WebThe BluePippin is a DNA size selection system from Sage Science. DNA size selection for next generation sequencing allows for separation from 100 bp – 50 kb using a pulsed-field electrophoresis. Target sizes or … WebThis instrument can used by customers to precisely size select DNA fragments of desired length. Computer controlled electrophoresis and sample gating permit fragments from …
Web5 rows · Jan 24, 2014 · Size-Select DNA Fragments up to 50kb Collect HMW DNA with High-Pass Filtering. The BluePippin ... BluePippin Support - BluePippin Sage Science Hls2 HMW Library System - BluePippin Sage Science SageELF - BluePippin Sage Science For proteomics users, SageELF delivers 12 size fractions in SDS buffer ready for … Video Guides - BluePippin Sage Science Sage Science develops products for the life sciences research markets. Our mission … Contact Us - BluePippin Sage Science Pippin Prep DNA Size Selection System: Request a quote: BLU0001: BluePippin … BEVERLY, Mass. — November 11, 2014 — Sage Science, a developer of life … Applications - BluePippin Sage Science
WebThe Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection. Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases. Gel cassettes available are: 3% agarose 100-250bp isolation. 2.5% agarose 100-600bp. 1.5% agarose 250bp – 1.5Kb. manhattan sx freesat hd box764/7019WebNov 9, 2024 · After library preparation, the library was run overnight for size selection using the Blue Pippin (Sage). The Blue Pippin was set to select a size range of 15–50 kbp. After collection of the ... manhattan supreme court justice michael katzWebThe BluePippin can be programmed to size select either targeted fractions or “High-Pass” collections. The fragment size distributions are described below: 1. Minimum Size … manhattan sx freesat remote controlWebApr 18, 2024 · After purification, size selection using a BluePippin Size selection system was performed (Sage Science, MA, USA) to enrich fragments longer than 4 kb. Then, an equal molar ratio mixed library of enriched fragments with those not processed by size selection was constructed using a SMRTbell Template Prep kit (Pacific Biosciences, … manhattan sweets bakery islip nyWebOct 27, 2016 · I am facing a problem with size selection of Covaris fragmented DNA. I would require insert sizes in the narrow range 700-800 bp for library preparation. BluePippin was not able to provide a narrow size range for the 750 bp library. So I was wondering if I can use the E-gel 2% size select for this narrow size selection. manhattan swivel chair palecekWebApr 11, 2024 · Gibberellins (GAs) are the key regulators controlling plant growth, wood production and the stress responses in perennial woody plants. The role of GA in regulating the above-mentioned processes in Eucalyptus remain largely unclear. There is still a lack of systematic identification and functional characterization of GA-related genes in … korean war and vietnam war differencesWebSize selection with the Blue Pippin high-pass collection methods eliminates the fragments below a specific cutoff. In this example, setting the size selection too small, such as 6 kb, is unlikely to eliminate much of the sample, while setting it too big, such as 20 kb, would eliminate almost all of the sample. Size selection for this sample manhattan sweets bakery islip